Feline Herpesvirus Type 1 Antigen Rapid Test (FHV-1 Ag)

Product specifications

Intended Use

The Feline Herpesvirus Type 1 Antigen Rapid Test is a lateral flow immunochromatographic assay for the qualitative detection of Feline Herpesvirus Type 1 antigen (FHV-1 Ag) in secretions from cat’s eyes, nasal cavities, and anus specimen, also from cat’s blood plasma or serum.

Assay Time

5-10 minutes

Principle

The Feline Herpesvirus Type 1 Antigen Rapid Test isbased on sandwich lateral flow immunochromatographic assay. The test device has atesting window for the observation of assay runningand result reading. The testing window has aninvisible T (test) zone and a C (control) zone beforerunning the assay. When the treated sample wasapplied into the sample hole on the device, the liquidwill laterally flow through the surface of the test stripand react with the pre-coated monoclonalantibodies. If there is FHV-1 antigen in the specimen,a visible T line will appear. The C line should alwaysappear after a sample is applied, which indicates avalid result. By this means, the device can accuratelyindicate the presence of feline Herpesvirus type 1antigen in the specimen.

Reagents and Materials

- Test devices, with disposable droppers

- Assay buffer

- Cotton swabs

- Products Manual

Storage and Stability

The kit can be stored at room temperature (4-30°C). The test kit is stable through the expiration date marked on the package label. DO NOT FREEZE. Do not store the test kit in direct sunlight.

Test Procedure

1. Collect cat’s ocular, nasal or anus secretions with the cotton swab and make the swab wet sufficiently.

2. Insert the swab into the provided assay buffer tube. Agitate it to get efficient sample extraction.

3. Take out the test device from the foil pouch and place it horizontally.

4. Suck the treated sample extraction from the assay buffer tube and place 3 drops into the sample hole“S” of the test device.

5. Interpret the result in 5-10 minutes. Result after 10minutes is considered as invalid.

Results Interpretation

- Positive (+): The presence of both “C” line and zone“T” line, no matter if “T” line is clear or vague.- Negative (-): Only clear C line appears. No “T” line.- Invalid: No coloured line appears in “C” zone. No matter if “T” line appears.

Precautions

- All reagents must be at room temperature beforerunning the assay.

- Do not remove test cassette from its pouch untilimmediately before use.

- Do not use the test beyond its expiration date.

- The components in this kit have been qualitycontrol tested as standard batch unit. Do not mixcomponents from different lot numbers.

- All specimens are of potential infection. It must betreated according to the local rules and regulations.

Limitations

Feline Herpesvirus Type 1 Antigen Rapid Test is for in vitro veterinary diagnosis use only. All results should be considered with other clinical information available from veterinarians. It is suggested to apply a further confirmative method when positive results are observed.

Download Data Sheet