Persecryl S-200

Persecryl S-200 is a hydrophilic gel filtration SEC resin with a particle size of 25–75 µm and a separation range of 5–250 kDa. It gives a high flow rate in column operation, with high resolution and recovery. The resin is used in the separation and purification of biological macromolecules such as enzymes, polysaccharides, nucleic acids and proteins, and in the production of biological products including interferon-γ, interleukin 2, protein A and hepatitis B vaccine.

Method of use

Chromatographic conditions

Buffer selection. Consider the stability of the sample in the buffer. Use a salt-containing buffer rather than ultrapure or pure water, to avoid non-specific adsorption.

Flow rate. Select a linear flow rate according to the column bed height, so that the maximum working pressure is not exceeded.

Sample pretreatment. Filter the sample through a 0.45 µm microporous membrane before loading to prevent the column from clogging.

Chromatography steps

1. Equilibration. Use the buffer to fully equilibrate the column until the pH and conductivity are stable and consistent with the equilibration buffer. This step usually requires 2–5 column bed volumes (CV).
2. Sample loading. The usual loading volume is 1–2% of the column volume. Do not use too high a sample concentration, to avoid overpressure and loss of resolution.
3. Elution. Elute with buffer and collect the peaks at different positions, usually over 1–1.5 CV.
4. Regeneration. Rinse the column with a high-salt buffer, such as 1M NaCl.
5. Re-equilibration. Re-equilibrate the column with buffer.

Cleaning and regeneration

Washing with buffer will usually restore equilibrium, after which the column can be used again. Some inactivated proteins and lipids cannot be washed out during regeneration and need to be removed by cleaning-in-place (CIP).

Clean lipids, inactivated proteins and precipitates with 0.2–0.5M NaOH or a non-ionic detergent at a flow rate of 15–20 cm/h, then wash with 2 CV of equilibration buffer. The whole cleaning process takes about 1–2 hours, depending on use.

Storage

Keep unopened resin in the original container at 4–30°C in a well-ventilated, dry and clean place. Do not freeze. Wash the used column with 2–3 CV of 20% ethanol solution and store at 2–8°C.

Destruction and recycling

Chromatography resin is difficult to degrade in nature, so incineration of waste resin is recommended. For resin that has been in contact with biologically active samples such as viruses and blood, follow local biosafety requirements before destroying or disposing of it.

Packing method

Detailed information on resin packaging is available on request. Please contact your local distributor.

Ordering information

• 200-00025 — 25 ml
• 200-00100 — 100 ml
• 200-00500 — 500 ml
• 200-01000 — 1 L
• 200-05000 — 5 L
• 200-10000 — 10 L

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