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Resin parameters

Matrix
Polystyrene
functional group
dT-20 mer
d50, µm
50
Dynamic Binding Capacity
≥2 mg mRNA /ml | 0.27 umol dT/ml
Recommended Flow Rate
1000 cm/h
Maximum Working Pressure
100 bar
maximum temperature
2–65°C
Compression factor
1.06
Ionic Strength
0 to 5 M common salt solutions

Chemical resistance

pH Stability
2-13
Avoid
Strong oxidizing agents (such as hypochlorite), oxidizing acids (such as nitric acid), strong reducing agents (such as sulfite), acetone, tetrahydrofuran or benzyl alcohol
* The physical and chemical properties and functions of the chromatographic resin have no obvious change after being placed in an environment of 40°C and pH 3–13 for 7 days.

Resin description

Helios Oligo dT affinity resin is a new type of high rigid polymer resin, which is mainly used for the separation and purification of mRNA. Helios Oligo dT is based on polystyrene, and the surface is covered with a large number of hydroxyl groups. The functionalized poly dT group can efficiently capture mRNA by base pairing with the poly-A tail of mRNA and improve production efficiency. Resin has high selectivity and extremely low non-specific adsorption.

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