DEAE Persedex A-25

DEAE Persedex A-25 is a weak anion-exchange IEX resin built on a highly cross-linked dextran matrix. It combines a high flow rate with low back pressure, high dynamic capacity and good chemical stability. The resin is convenient to scale up, shortening production time, improving production efficiency and reducing the cost of large-scale production.

Method of use

DEAE Persedex A-25 is supplied as a dry powder and must be swollen before use. The swelling ratio depends on the buffer used and differs considerably between solutions. Do not use magnetic stirring during swelling, as this easily breaks the gel.

Chromatography steps

1. Pre-treatment. Weigh out the required amount of DEAE Persedex A-25, suspend it in 50–100 volumes of distilled water or loading equilibration buffer and allow it to swell. Swelling usually takes 1–2 days at room temperature, or 2 hours in boiling water.
2. Column packing. Pack the column according to standard operating procedure. Ensure that all materials are at working temperature and degas the gel before packing.
3. Equilibration. Equilibrate the column with 2–5 column bed volumes (CV), making sure that the conductivity and pH of the eluent are exactly the same as those of the loading buffer.
4. Sample loading. Determine the loading amount from the target product concentration and the loading capacity of the gel.
5. Washing. After loading the sample, equilibrate the column with loading buffer to wash away unbound proteins and impurities, until the conductivity and pH of the eluent are exactly the same as those of the loading buffer.
6. Elution. Use sequential or gradient elution, either by increasing the salt concentration of the buffer or by decreasing the pH.
7. Regeneration. Wash the impurity proteins off the column with 1–2M NaCl, then wash the salt out of the column with distilled water until the conductivity and pH of the eluent stabilise. Finally, run loading buffer through the column until the conductivity and pH of the eluent are stable.

Storage

Keep unopened resin in the original container at 4–30°C in a well-ventilated, dry and clean place. Do not freeze. Wash the used column with 2–3 CV of 20% ethanol solution and store at 2–8°C.

Destruction and recycling

Chromatography resin is difficult to degrade in nature, so incineration of waste resin is recommended to protect the environment. For resin that has been in contact with biologically active samples such as viruses and blood, follow local biosafety requirements before destroying or disposing of it.

Packing method

Detailed information on resin packaging is available on request. Please contact your local distributor.

Ordering information

• 261-00025 — 25 g
• 261-00100 — 100 g
• 261-00500 — 500 g
• 261-01000 — 1 kg
• 261-05000 — 5 kg
• 261-10000 — 10 kg

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