Aether Q

Aether Q IEX resin is based on polyacrylate, and the surface is hydrophilically modified with neutral polymers which are then bonded with strong acidic ion-exchange groups. This chromatographic resin has ultra-high dynamic loading. It can withstand high ionic strength, has excellent biocompatibility and column bed stability, and can provide faster flow rate and improve purification efficiency. At the same time, the superior alkali resistance can prolong the service life, improve production efficiency, reduce production costs and create better economic benefits. It is widely used in capture and purification.

Method of use

Equilibration

Equilibrate the column with an appropriate 5–10 CV buffer until the conductivity and pH of the eluent remain unchanged, consistent with the equilibration solution. For example, the equilibration buffer could be 20 mM PBS, pH 7.0. Screening and optimisation should be carried out according to the stability and isoelectric point of the target protein, and the type of ion-exchange resin.

Sample loading

Solid samples can be prepared by dissolving in equilibration buffer. Low-concentration sample solutions can be dialysed with equilibration buffer; high-concentration sample solutions can be diluted with equilibration buffer. To avoid column clogging, samples should be centrifuged or membrane filtered, preferably 0.45 or 0.22 µm.

The amount of feed is calculated according to the loading capacity of the resin and the content of the target protein in the feed solution. Before loading the sample, ensure that the sample buffer is as consistent as possible with the equilibration buffer.

Elution

After loading the sample, continue to rinse with the equilibration buffer until the baseline is stable. According to the situation, the method of increasing the salt concentration or changing the pH of the mobile phase can be used to elute the samples adsorbed on the chromatographic resin in sequence.

Regeneration

After each chromatography, the column should be washed with 0.5–2M NaCl to remove proteins strongly bound to the chromatography resin.

Cleaning and regeneration

Contaminants (e.g. lipids, endotoxins and proteins) accumulate on the column as the number of uses of the chromatography resin increases. Determine the frequency of CIP according to the degree of contamination of the chromatography resin. Where contamination is considerable, CIP after each use is recommended to ensure repeatability of results and to prolong the working life of the chromatography resin.

For different types of impurities and contaminants, the recommended cleaning conditions are as follows:

• Strongly binding proteins: wash with 5 CV of 2M NaCl solution, or use a high salt buffer not lower than pH 2, such as 1M NaAc solution.
• Strongly hydrophobic proteins and precipitated proteins: first wash with 5 CV of 0.2–0.5M NaOH solution (contact time 1–2 hours), then wash out the lye with 5–10 CV of ultrapure or pure water.
• Lipoproteins and lipids: first wash with 5 CV of 50% ethanol or 30% isopropanol (contact time 0.5–1 hour), then rinse with 5–10 CV of ultrapure or pure water. It can also be cleaned with an alkaline or acidic solution containing a non-ionic surfactant, such as 0.1~0.5% Triton X-100 + 0.1M acetic acid for 12 hours, then rinsed with more than 5 CV of 50% ethanol to remove the detergent, and rinsed with 5 CV of ultrapure or pure water as above. When using high-concentration organic solvents, increase the concentration gradually to avoid air bubbles.

Degas 50% ethanol or 30% isopropanol before use. Use a flow rate of 30–60 cm/h during CIP. Use reverse cleaning where the clogging is severe.

To reduce the microbial load, it is recommended that 0.5~1M NaOH solution is used to treat the chromatographic resin.

Storage

Keep the unopened chromatography resin in the original container and store at 4~30°C in a well-ventilated, dry and clean place. Do not freeze. Wash the used column with 2–3 CV of 20% ethanol solution and store at 2~8°C.

Destruction and recycling

Since chromatography resin is difficult to degrade in nature, it is recommended that the waste chromatography resin is incinerated to protect the environment. For chromatography resin that has been in contact with biologically active samples such as viruses and blood, follow the local biosafety requirements before destroying or disposing of it.

Packing method

Detailed information on resin packaging is available on request. Please contact your local distributor.

Ordering information

• 232-00025 — 25 ml
• 232-00100 — 100 ml
• 232-00500 — 500 ml
• 232-01000 — 1 L
• 232-05000 — 5 L
• 232-10000 — 10 L