Trypsin-EDTA (0.05 %) in HBSS (1x), with phenol red

Trypsin-EDTA solutions are used to detach adherent cells from culture surfaces. They are composed of natural porcine pancreas-derived trypsin and EDTA. The addition of phenol red serves as pH indicator to adjust to the perfect cell culture conditions.

The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Various formulations should be tested to determine the best product for a specific application.

Store at ≤-15 °C. Avoid repeated freeze-thaw cycles. Preparation of aliquots recommended. Once opened, store at 4 °C and use within 2-4 weeks. Thawing: +37 °C water bath or overnight at +2 °C to +8 °C. Swirl gently to homogenize.