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Product Specifications

Appearance

Clear light yellow liquid

Shipping Conditions

Ambient

CO2 Concentration, Optimum

0.0 – 0.5 %

Description

Dr. Gardiner and Dr. Stockdale developed TC-100 Insect medium in the mid-1970s by modifying Grace's medium. The purpose of the modification was to enhance the production of Autographa californica NPV virions by cells from the fall armyworm, Spodoptera frugiperda. TC-100 Insect medium can support the growth of cells derived from various lepidopteran species, including Sf9, when supplemented with 10% fetal bovine serum (FBS). This medium is primarily used for the production of baculoviruses in lepidopteran cell lines.

Applications: Insect cell culture (e.g. Sf9); Production of Baculovirus

Storage and Shelf Life

Store at +2 °C to +8 °C, protected from light. Once opened store at 4 °C and use within 6-8 weeks.

Download Data Sheet

Formulation

Amino Acids

Glycine 650.00 mg/l
L-Alanine 225.00 mg/l
L-Arginine HCl 700.00 mg/l
L-Asparagine 350.00 mg/l
L-Aspartic Acid 350.00 mg/l
L-Cystine 2 HCl 25.00 mg/l
L-Glutamic Acid 600.00 mg/l
L-Glutamine 600.00 mg/l
L-Histidine HCl H2O 3086.34 mg/l
L-Isoleucine 50.00 mg/l
L-Leucine 75.00 mg/l
L-Lysine HCl 625.00 mg/l
L-Methionine 50.00 mg/l
L-Phenylalanine 150.00 mg/l
L-Proline 350.00 mg/l
L-Serine 550.00 mg/l
L-Threonine 175.00 mg/l
L-Tryptophan 100.00 mg/l
L-Tyrosine disodium salt 62.09 mg/l
L-Valine 100.00 mg/l

Vitamins

Choline Chloride 0.20 mg/l
D-Biotin 0.01 mg/l
D-Calcium Pantothenate 0.02 mg/l
Folic Acid 0.02 mg/l
Nicotinic Acid (Niacin) 0.02 mg/l
Pyridoxine HCl 0.02 mg/l
Riboflavin 0.02 mg/l
Thiamine HCl 0.52 mg/l
myo-Inositol 0.02 mg/l
p-Amino Benzoic Acid 0.02 mg/l

Inorganic Salts

CaCl2 2 H2O 1319.82 mg/l
MgCl2 1068.20 mg/l
MgSO4 1357.63 mg/l
NaH2PO4 876.92 mg/l
KCl 2870.00 mg/l
NaHCO3 350.00 mg/l

Other Components

D-Glucose 1000.00 mg/l
Tryptose Broth 2600.00 mg/l

Precautions and Disclaimer

This product is for research use and further manufacturing only.

TC100 Insect Medium, with L-Glutamine