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SILAC RPMI 1640, w/o L-Arginine, w/o L-Lysine, w/o L-Glutamine, w/o Phenol Red
The SILAC RPMI medium has been designed and optimised for labeling experiments that involve the use of stable amino acid isotopes in cell culture for the purpose of analyzing protein expression through mass spectrometry (MS).
Product Specifications
Appearance
Clear solution
Shipping Conditions
Ambient
CO2 Concentration, Optimum
4.5 %
Description
Applications:
- Quantitative and functional proteomics
- Analyses of tissue regeneration
- Analyses of post-translational modifications
- MS (Mass Spectrometry)
- NMR (Nuclear Magnetic Resonance)
Storage and Shelf Life
Store at +2 °C to +8 °C protected from light. Once opened, store at 4 °C and use within 6-8 weeks.
Formulation
Amino Acids
- Glycine 10.00 mg/l
- L-Asparagine H2O 56.82 mg/l
- L-Aspartic Acid 20.00 mg/l
- L-Cystine 2HCl 65.20 mg/l
- L-Glutamic Acid 20.00 mg/l
- L-Histidine HCl H2O 20.27 mg/l
- L-Hydroxy-L-Proline 20.00 mg/l
- L-Isoleucine 50.00 mg/l
- L-Leucine 50.00 mg/l
- L-Methionine 15.00 mg/l
- L-Phenylalanine 15.00 mg/l
- L-Proline 20.00 mg/l
- L-Serine 30.00 mg/l
- L-Threonine 20.00 mg/l
- L-Tryptophan 5.00 mg/l
- L-Tyrosine 2Na 2H2O 28.83 mg/l
- L-Valine 20.00 mg/l
Vitamins
- p-Amino Benzoic Acid 1.00 mg/l
- D-Biotin 0.20 mg/l
- Choline Chloride 3.00 mg/l
- D-Calcium Pantothenate 0.25 mg/l
- Folic Acid 1.00 mg/l
- myo-Inositol 35.00 mg/l
- Nicotinamide 1.00 mg/l
- Pyridoxine HCl 1.00 mg/l
- Riboflavin 0.20 mg/l
- Thiamine HCl 1.00 mg/l
- Vitamin B12 0.005 mg/l
Inorganic Salts
- Ca(NO3)2 4 H2O 100.00 mg/l
- KCl 400.00 mg/l
- MgSO4 7H2O 100.00 mg/l
- NaCl 6000.00 mg/l
- NaHCO3 2000.00 mg/l
- Na2HPO4 800.00 mg/l
Other Components
- D-Glucose 2000.00 mg/l
- L-Glutathione Reduced 1.00 mg/l
Precautions and Disclaimer
This product is for research use only.