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SILAC DMEM, high Glucose (4.5 g/L), w/o L-Arginine, w/o L-Lysine, w/o L-Glutamine, w/o Phenol Red
The SILAC DMEM medium has been optimized for conducting labeling experiments in cell cultures using stable amino acid isotopes. These experiments are used to analyze protein expression through mass spectrometry (MS).
Product Specifications
Appearance
Clear solution
Shipping Conditions
Ambient
CO2 Concentration, Optimum
8.5%
Description
Applications:
- Quantitative and functional proteomics
- Analyses of tissue regeneration
- Analyses of post-translational modifications
- MS (Mass Spectrometry)
- NMR (Nuclear Magnetic Resonance)
Storage and Shelf Life
Store at +2 °C to +8 °C protected from light. Once opened, store at 4 °C and use within 6-8 weeks.
Formulation
Amino Acids
- Glycine 30.00 mg/l
- L-Cystine 2 HCl 62.57 mg/l
- L-Histidine HCl H2O 42.00 mg/l
- L-Isoleucine 105.00 mg/l
- L-Leucine 105.00 mg/l
- L-Methionine 30.00 mg/l
- L-Phenylalanine 66.00 mg/l
- L-Serine 42.00 mg/l
- L-Threonine 95.00 mg/l
- L-Tryptophan 16.00 mg/l
- L-Tyrosine 2 Na 2 H2O 103.79 mg/l
- L-Valine 94.00 mg/l
Vitamins
- Choline chloride 4.00 mg/l
- D-Calcium Pantothenate 4.00 mg/l
- Folic Acid 4.00 mg/l
- myo-Inositol 7.20 mg/l
- Nicotinamide 4.00 mg/l
- Pyridoxal HCl 4.00 mg/l
- Riboflavin 0.40 mg/l
- Thiamine HCl 4.00 mg/l
Inorganic Salts
- CaCl2 2 H2O 265.00 mg/l
- Fe(NO3)3 9 H2O 0.10 mg/l
- KCl 400.00 mg/l
- MgSO4 7 H2O 200.00 mg/l
- NaCl 6400.00 mg/l
- NaHCO3 3700.00 mg/l
- NaH2PO4 2 H2O 141.73 mg/l
Other Components
- D-Glucose 4500.00 mg/l
Precautions and Disclaimer
This product is for research use only.