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RPMI 1640, with L-Glutamine, powder medium, with 25 mM HEPES, w/o Sodium Bicarbonate
With its unique composition containing high levels of vitamins and glutathione, RPMI 1640 medium is well-suited for promoting the growth of a wide range of cell types when compared to other media.
Product Specifications
pH
6.40 – 7.00 (w/o NaHCO₃), 6.70 – 7.30 (w/NaHCO₃)
Osmolality
280 – 320 mOsm/kg (w/o NaHCO₃), 320 – 360 mOsm/kg (w/NaHCO₃)
Endotoxin
< 5 EU/ml
Sterility
Tested
Appearance
Off-white to creamy white, homogenous powder
CO2 Concentration, Optimum
4.5%
Solubility
Clear solution at 16.4 g/l
Use At
16.4 g/l
Add
2.0 g/l Sodium Bicarbonate
Storage and Shelf Life
+2 °C to +8 °C, dry and protected from light
Instructions
Preparation of 1 liter liquid medium:
- Suspend 10.0 g in 900 ml tissue culture grade water with constant, gentle stirring until the powder is completely dissolved. Do not heat the water.
- Add 3.7 g of sodium bicarbonate powder or 49.3 ml of 7.5 % sodium bicarbonate solution for 1 liter of medium and stir until dissolved.
- Adjust the pH to 0.2 to 0.3 pH units below the desired pH using 1 N HCl or 1 N NaOH since the pH tends to rise during filtration.
- Make up the final volume to 1000 ml with tissue culture grade water.
- Sterilize the medium immediately by filtering through a sterile membrane filter with porosity of 0.22 micron or less, using positive pressure rather than vacuum to minimize the loss of carbon dioxide.
- Aseptically add sterile supplements as required and dispense the desired amount of sterile medium into sterile containers.
- Store liquid medium at +2 °C to +8 °C and in dark until use.
Formulation
Amino Acids
- L-Arginine HCl 241.00 mg/l
- L-Asparagine 50.00 mg/l
- L-Aspartic Acid 20.00 mg/l
- L-Cystine x 2 HCl 65.20 mg/l
- L-Glutamine 300.00 mg/l
- Glycine 10.00 mg/l
- L-Glutamic Acid 20.00 mg/l
- L-Histidine HCl 20.96 mg/l
- L-Hydroxy-L-Proline 20.00 mg/l
- L-Isoleucine 50.00 mg/l
- L-Leucine 50.00 mg/l
- L-Lysine x HCl 40.00 mg/l
- L-Methionine 15.00 mg/l
- L-Phenylalanine 15.00 mg/l
- L-Proline 20.00 mg/l
- L-Serine 30.00 mg/l
- L-Threonine 20.00 mg/l
- L-Tryptophan 5.00 mg/l
- L-Tyrosine x 2 Na 2 H2O 28.83 mg/l
- L-Valine 20.00 mg/l
Vitamins
- p-Amino Benzoic Acid 1.00 mg/l
- D-Biotin 0.20 mg/l
- Choline Chloride 3.00 mg/l
- D-Calcium Pantothenate 0.25 mg/l
- Folic Acid 1.00 mg/l
- myo-Inositol 35.00 mg/l
- Nicotinamide 1.00 mg/l
- Pyridoxine x HCl 1.00 mg/l
- Riboflavin 0.20 mg/l
- Thiamine x HCl 1.00 mg/l
- Vitamin B12 0.005 mg/l
Inorganic Salts
- Calcium Nitrate x 4 H2O 100.00 mg/l
- Magnesium Sulfate 48.84 mg/l
- Potassium Chloride 400.00 mg/l
- Sodium Chloride 6000.00 mg/l
- Sodium Phosphate Dibasic Anhydrous 800.00 mg/l
Other Components
- D-Glucose 2000.00 mg/l
- L-Glutathione Reduced 1.00 mg/l
- HEPES 5958.00 mg/l
- Phenol Red Sodium Salt 5.30 mg/l
Precautions and Disclaimer
This product is for research use only. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.