RPMI 1640, w/o L-Glutamine, with 25 mM HEPES

Amino Acids

• Glycine 10.00 mg/l
• L-Arginine 200.00 mg/l
• L-Asparagine 50.00 mg/l
• L-Aspartic Acid 20.00 mg/l
• L-Cystine 2HCl 65.20 mg/l
• L-Glutamic Acid 20.00 mg/l
• L-Histidine 15.00 mg/l
• L-Hydroxy-L-Proline 20.00 mg/l
• L-Isoleucine 50.00 mg/l
• L-Leucine 50.00 mg/l
• L-Lysine HCl 40.00 mg/l
• L-Methionine 15.00 mg/l
• L-Phenylalanine 15.00 mg/l
• L-Proline 20.00 mg/l
• L-Serine 30.00 mg/l
• L-Threonine 20.00 mg/l
• L-Tryptophan 5.00 mg/l
• L-Tyrosine 2Na 2H2O 28.83 mg/l
• L-Valine 20.00 mg/l

Vitamins

• p-Amino Benzoic Acid 1.00 mg/l
• D-Biotin 0.20 mg/l
• Choline Chloride 3.00 mg/l
• D-Calcium Pantothenate 0.25 mg/l
• Folic Acid 1.00 mg/l
• myo-Inositol 35.00 mg/l
• Nicotinamide 1.00 mg/l
• Pyridoxine HCl 1.00 mg/l
• Riboflavin 0.20 mg/l
• Thiamine HCl 1.00 mg/l
• Vitamin B12 0.005 mg/l

Inorganic Salts

• Ca(NO3)2·4 H2O 100.00 mg/l
• KCl 400.00 mg/l
• MgSO4 48.84 mg/l
• NaCl 6000.00 mg/l
• NaHCO3 2000.00 mg/l
• Na2HPO4 800.00 mg/l

Other Components

• D-Glucose 2000.00 mg/l
• HEPES 5958.00 mg/l
• L-Glutathione Reduced 1.00 mg/l
• Phenol Red Sodium Salt 5.30 mg/l