MycoXpert, Mycoplasma Removal Reagent (50x)

Mycoplasma, first discovered in 1898, was firstly classified as virus. There are more than 100 known species belonging to the genus Mycoplasma. They are the smallest free-living, self-replicating organisms and lack a cell wall so that antibiotics such as penicillin, which interfere with murein formation, are not effective. If not treated, mycoplasma contaminations can lead to diminished cell growth and to the loss of cultures.

Procedure

Treatment with Mycoplasma Removal Reagent includes three or more cycles (passages). It is recommended to check the culture every week for presence of mycoplasma (e.g. ELISA, fluorescence staining or PCR). Please note, that using PCR for detection may lead to false positive results during treatment with Mycoplasma Removal Reagent since this method cannot distinguish between living and dead mycoplasma. We recommend using PCR as detection method only approx. 2 weeks after treatment with Mycoplasma Removal Reagent in order to avoid false positive results.

Normally mycoplasma will be removed after 2 to 3 weeks.

Store at ≤-15 °C. Avoid repeated freeze-thaw cycles.