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MEM Alpha Modification, w/o L-Glutamine, w/o Nucleosides
MEM is a commonly utilized cell culture medium that can support the growth of various cell types, whether they are adherent or suspension.
Product Specifications
pH
7.0 – 7.5
Osmolality
260 – 340 mOsm/kg
Endotoxin
<1 EU/ml (<0.1 ng/ml)
Sterility
Tested
Appearance
Clear red orange solution
CO2 Concentration, Optimum
5.0%
Storage and Shelf Life
+2 °C to +8 °C protected from light, do not freeze!
Formulation
Amino Acids
- L-Alanine 25.00 mg/l
- L-Arginine · HCl 126.00 mg/l
- L-Asparagine · H2O 50.00 mg/l
- L-Aspartic Acid 30.00 mg/l
- L-Cysteine · HCl · H2O 100.00 mg/l
- L-Cystine · 2HCl 31.30 mg/l
- L-Glutamic Acid 75.00 mg/l
- Glycine 50.00 mg/l
- L-Histidine · HCl · H2O 42.00 mg/l
- L-Isoleucine 52.00 mg/l
- L-Leucine 52.00 mg/l
- L-Lysine · HCl 72.50 mg/l
- L-Methionine 15.00 mg/l
- L-Phenylalanine 32.00 mg/l
- L-Proline 40.00 mg/l
- L-Serine 25.00 mg/l
- L-Threonine 48.00 mg/l
- L-Tryptophan 10.00 mg/l
- L-Tyrosine · 2 Na · H2O 51.90 mg/l
- L-Valine 46.00 mg/l
Vitamins
- Ascorbic Acid · Na 50.00 mg/l
- D-Biotin 0.10 mg/l
- Choline chloride 1.00 mg/l
- D-Calcium Pantothenate 1.00 mg/l
- Folic Acid 1.00 mg/l
- myo-Inositol 2.00 mg/l
- Nicotinamide 1.00 mg/l
- Pyridoxal HCl 1.00 mg/l
- Riboflavin 0.10 mg/l
- Thiamine HCl 1.00 mg/l
- Vitamin B12 1.36 mg/l
Inorganic Salts
- CaCl2 · 2 H2O 200.00 mg/l
- MgSO4 (anhydrous) 97.67 mg/l
- KCl 400.00 mg/l
- NaHCO3 2200.00 mg/l
- NaCl 6800.00 mg/l
- Na2HPO4 (anhydrous) 122.00 mg/l
Other Components
- D-Glucose 1000.00 mg/l
- Lipoic Acid 0.20 mg/l
- Phenol Red · Na 11.00 mg/l
- Sodium Pyruvate 110.00 mg/l
Precautions and Disclaimer
This product is for research use only. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.