Product Specifications
Description
The medium is supplied frozen as a complete medium, ready to use in a 100 ml format. It is based on MEM Alpha Modification and contains antibiotics (gentamycin), L-glutamine, fetal bovine serum (FBS), hormones and growth factors. It is buffered with sodium bicarbonate and phenol red is present as a pH indicator.
Thawing
Thaw MarrowFirst Medium at +37 °C in a water bath and mix gently during and after thawing to obtain a homogeneous medium.
An alternative is to thaw medium in a +37 °C CO2 incubator with the lid slightly opened to allow automatic pH normalization. Warm medium at the appropriate pH is best for the initialization of cultures.
Applications and Features
- For Karyotyping, fluorescence in-situ hybridization and other cytogenetic procedures of established bone marrow and leukemic blood cell cultures.
Storage and Shelf Life
Store at ≤-15 °C protected from light. Do not use this product after its expiry date. Once opened, store at +2 °C to +8 °C and use within 2 weeks.

