Lymphocyte Separation Medium, Density 1.077 g/ml | LT Biotech EU-Grade Cell Culture Reagents

Request Sample

Product Specifications

6.7 – 7.4

Sterility

Tested

Description

Protocol for use: Separation of Lymphocytes from Whole Blood

According to the blood volume to be separated, either 15 or 25 ml size centrifuge tubes are filled with either 7 or 10 ml Lymphocyte Separation Medium (D = 1.077 g/ml, at +20 °C). Heparinized whole blood is then, either undiluted or diluted with equal parts of PBS or culture medium, carefully poured over the lymphocyte separation solution. The separation process is performed by centrifugation at 1200 g for 20 minutes.

The lymphocytes (70 % to 100 % enrichment) concentrate in the interphase (white layer) between the plasma and the separation solution (Fig. 1).

Applications and Features

Viability of cells and cell separation - Tested

Storage and Shelf Life

Shipped at ambient temperature. Upon receipt store at +2 °C to +8 °C. Note: Lymphocyte Separation Medium is light sensitive. The medium should be protected from light during shipping and storage.

Download Data Sheet

Precautions and Disclaimer

For research use only. Not for use in diagnostic procedures. According to the blood volume to be separated, either 15 or 25 ml size centrifuge tubes are filled with either 7 or 10 ml Lymphocyte Separation Medium (D = 1.077 g/ml, at +20 °C). Heparinized whole blood is then, either undiluted or diluted with equal parts of PBS or culture medium, carefully poured over the lymphocyte separation solution. The separation process is performed by centrifugation at 1200 g for 20 minutes. The lymphocytes (70 % to 100 % enrichment) concentrate in the interphase (white layer) between the plasma and the separation solution (Fig. 1).