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Product Specifications

Appearance

Clear red orange solution

Shipping Conditions

Ambient

CO2 Concentration, Optimum

0.0%

Description

Leibovitz's L-15 Medium was initially developed for use in CO2-free environments and therefore does not include sodium bicarbonate in its formulation. Instead, the medium's pH is buffered through the use of salts, freebase amino acids, and galactose as a substitute for glucose. This helps to maintain the physiological pH of the solution. Leibovitz's L-15 Medium is capable of supporting various cell lines, such as HEp-2 and LLC-MK2, as well as primary embryonic and human tissue cultures.

Reconstitution: Usually the medium is supplemented with 0.3 g/l L-glutamine (2 mM).

Storage and Shelf Life

Store at +2 °C –+8 °C protected from light. Once opened, store at +2 °C to +8 °C and use within 6-8 weeks.

Download Data Sheet

Formulation

Amino Acids

Glycine 200.00 mg/l
L-Alanine 225.00 mg/l
L-Arginine 500.00 mg/l
L-Asparagine 250.00 mg/l
L-Cysteine 120.00 mg/l
L-Histidine 250.00 mg/l
L-Isoleucine 125.00 mg/l
L-Leucine 125.00 mg/l
L-Lysine·HCl 93.70 mg/l
L-Methionine 75.00 mg/l
L-Phenylalanine 125.00 mg/l
L-Serine 200.00 mg/l
L-Threonine 300.00 mg/l
L-Tryptophan 20.00 mg/l
L-Tyrosine 300.00 mg/l
L-Valine 100.00 mg/l

Vitamins

Choline chloride 1.00 mg/l
D-Calcium Pantothenate 1.00 mg/l
Folic Acid 1.00 mg/l
myo-Inositol 2.00 mg/l
Nicotinamide 1.00 mg/l
Pyridoxal HCl 1.00 mg/l
Riboflavin 0.10 mg/l
Thiamine Monophosphate HCl 1.00 mg/l

Inorganic Salts

CaCl2 2 H2O 185.00 mg/l
KCl 400.00 mg/l
KH2PO4 (anhydrous) 60.00 mg/l
MgCl ·6H2O 200.00 mg/l
MgSO4 (anhydrous) 97.67 mg/l
NaCl 8000.00 mg/l
Na2HPO4 (anhydrous) 190.00 mg/l

Other Components

D-Galactose 900.00 mg/l
Phenol Red·Na 11.00 mg/l
Sodium Pyruvate 550.00 mg/l

Precautions and Disclaimer

This product is for research use only.

Leibovitz's L-15 Medium, w/o L-Glutamine