Cell Growth Additives

Hybridoma Supplement, Serum-free

Hybridoma cells result from fusion of an antibody producing B-cell from the immune system with a tumor cell. To sup- port hybridoma development and achieve an optimal cell density as well as cloning efficiency growth factors and serum (10 to 20 %) are required. Hybridoma Supplement is a chemically defined growth promoting supplement containing in- sulin, ethanolamine, hydrocortisone, retinoic acid, linoleic acid and other growth promoting factors.

Product Specifications

Description

As supplement to culture medium it supports the growth of hybridoma in a manner feeder cells have been used before. The disadvantages occurring by the use of feeder cells may include: overgrowth of newly formed hybridomas; source of contamination, competition for nutrients, and variations in growth factor concentrations.

Hybridoma Supplement can be used in culture medium under serum-free conditions. The low protein content facilitates the isolation and purification of produced antibodies. Hybridoma Supplement is provided as a 10x concentrate.

Applications and Features

Improvement of Hybridoma Growth after Fusion.
Improvement of cloning efficiency of hybridomas.
Production of Monoclonal Antibodies under serum-free conditions.

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Precautions and Disclaimer

This product is for research use only. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.