G-418 Sulfate, Solution, 100 % Activity (50 mg/ml)

Important Information

• Do not use G-418 with antibiotic/antifungal preparations (e.g. Pen/Strep). These agents are competitive inhibitors of G-418. Other antibiotics are potentially cross-reactive as
• Good laboratory practice requires that the optimal concentration of biologically active G-418 to select and maintain cells must be determined for each set of growth G-418 is used in the concentration range of 100 – 200 μg/ml for bacteria, or 200 – 500 μg/ml for most mammalian cells. Concentrations of G-418 required for maintenance of selected cell lines are typically ≤ 50% compared to selection.
• It is recommended that whenever experimental conditions are altered, the optimal concentration of the product should be re-evaluated.

Store at ≤-15 °C. Avoid repeated freeze-thaw cycles. Preparation of aliquots recommended. Once opened, store at +4 °C and use within 4-6 weeks. Thawing: Overnight at +2 °C to +8 °C. Swirl gently to homogenize.