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G-418 Sulfate, Powder
G-418 is used in the selection and maintenance of eucaryotic cells stably transfected with neomycin resistance genes. G-418 is an aminoglycoside antibiotic, related to Gentamicin, and exhibits toxicity towards both eukaryotic and prokaryotic cells. It is produced by Micromonospora rhodorangea and acts by binding the ribosome, thus inhibiting protein synthesis in both prokaryotic and eukaryotic cells.
Product Specifications
Appearance
White or off-white powder
Shipping Conditions
Ambient
Working Concentration
Recommended final concentration (0.1 – 1.0 mg/ml) depending on the cell type: HeLa: 200 – 600 µg/ml; 3T3 cells: 500 – 1000 µg/ml; CHO: 200 – 400 µg/ml; HEK 293: 500 – 800 µg/ml; Jurkat cells: 600 – 700 µg/ml
Storage and Shelf Life
Store at +2 – +8 °C.
Precautions and Disclaimer
This product is for research use only.