Fetal Bovine Serum, Heat Inactivated, USA

6.8 – 8.2

240 – 340 mOsm/kg

As reported

3.0 – 4.5 g/dl

As reported

As reported

Not detected

PI-3, BVDV, BVDV-AB, BHV-I

Tested

Heat inactivation will inactivate the complement system, antibodies and other active enzymes. It has to be done in a carefully controlled process in order to avoid damaging the cell growth promoting properties of the serum and reducing the formation of unwanted precipitates. The process involves heating the serum in a shaking water bath at exactly +56°C for 30 minutes. The shaking will help avoid the formation of protein and other forms of precipitates. After 30 minutes the serum is then cooled back down to room temperature as quickly as possible to avoid excessive exposure to heat which can damage e.g. growth factors and vitamins.

LT Biotech has established process parameters and controls for maximum inactivation of contaminants by an innovative gamma irradiation process in small sized boxes at 25 – 35kGy. When FBS bottles are arranged in a pallet during gamma irradiation, high irradiation doses (58 kGy) are necessary to irradiate the centrally located bottles with the required does (>30 kGy). This and the associated higher temperatures may affect the serum quality. If the irradiation is performed in single boxes, the maximum does of irradiation is performed in single boxes, the maximum dose of irradiation does not exceed 38.7 kGy. Gentle irradiation as used by LT Biotech is less likely to affect final serum quality.