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DMEM High Glucose (4.5 g/l) powder medium, with L-Glutamine, with Sodium Pyruvate, w/o Sodium Bicarbonate
Product Specifications
Storage and Shelf Life
+2 °C to +8 °C, dry and protected from light
Instructions
Preparation of 1 liter liquid medium:
- Suspend 13.5 g in 900 ml tissue culture grade water with constant, gentle stirring until the powder is completely dissolved. Do not heat the water.
- Add 3.7 g of sodium bicarbonate powder or 49.3 ml of 7.5 % sodium bicarbonate solution for 1 liter of medium and stir until dissolved.
- Adjust the pH to 0.2 to 0.3 pH units below the desired pH using 1 N HCl or 1 N NaOH since the pH tends to rise during filtration.
- Make up the final volume to 1000 ml with tissue culture grade water.
- Sterilize the medium immediately by filtering through a sterile membrane filter with porosity of 0.22 micron or less, using positive pressure rather than vacuum to minimize the loss of carbon dioxide.
- Aseptically add sterile supplements as required and dispense the desired amount of sterile medium into sterile containers.
- Store liquid medium at +2 °C to +8 °C and in dark until use.
Formulation
Amino Acids
- L-Arginine x HCl 84.00 mg/l
- L-Cystine x 2 HCl 62.57 mg/l
- L-Glutamine 584.00 mg/l
- Glycine 30.00 mg/l
- L-Histidine x HCl x H2O 42.00 mg/l
- L-Hydroxy-L-Proline 20.00 mg/l
- L-Isoleucine 105.00 mg/l
- L-Leucine 105.00 mg/l
- L-Lysine x HCl 146.00 mg/l
- L-Methionine 30.00 mg/l
- L-Phenylalanine 66.00 mg/l
- L-Proline 20.00 mg/l
- L-Serine 42.00 mg/l
- L-Threonine 95.00 mg/l
- L-Tryptophan 16.00 mg/l
- L-Tyrosine x 2 Na 103.79 mg/l
- L-Valine 94.00 mg/l
Vitamins
- Choline Chloride 4.00 mg/l
- D-Calcium Pantothenate 4.00 mg/l
- Folic Acid 4.00 mg/l
- myo-Inositol 7.20 mg/l
- Nicotinamide 4.00 mg/l
- Pyridoxal x HCl 4.00 mg/l
- Riboflavin 0.40 mg/l
- Thiamine x HCl 4.00 mg/l
Inorganic Salts
- Calcium Chloride x 2 H2O 265.00 mg/l
- Ferric Nitrate x 9 H2O 0.10 mg/l
- Magnesium Sulfate Anhydrous 97.72 mg/l
- Potassium Chloride 400.00 mg/l
- Sodium Chloride 6400.00 mg/l
- Sodium Dihydrogen Phosphate Anhydrous 109.00 mg/l
Other Components
- D-Glucose 4500.00 mg/l
- Phenol Red Sodium Salt 15.90 mg/l
- Sodium Pyruvate 110.00 mg/l
Precautions and Disclaimer
Here are some frequently asked questions
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