DMEM/Ham's F-12, w/o L-Glutamine, with 15 mM HEPES

Amino Acids

• Glycine 18.75 mg/l
• L-Alanine 4.45 mg/l
• L-Arginine HCl 147.50 mg/l
• L-Asparagine H2O 7.50 mg/l
• L-Aspartic Acid 6.65 mg/l
• L-Cysteine HCl H2O 17.56 mg/l
• L-Cystine 2 HCl 31.29 mg/l
• L-Glutamic Acid 7.35 mg/l
• L-Glutamine 365.00 mg/l
• L-Histidine HCl H2O 31.48 mg/l
• L-Isoleucine 54.47 mg/l
• L-Leucine 59.05 mg/l
• L-Lysine HCl 91.25 mg/l
• L-Methionine 17.24 mg/l
• L-Phenylalanine 35.48 mg/l
• L-Proline 17.25 mg/l
• L-Serine 26.25 mg/l
• L-Threonine 53.45 mg/l
• L-Tryptophan 9.02 mg/l
• L-Tyrosine 2 Na 2 H2O 55.79 mg/l
• L-Valine 52.85 mg/l

Vitamins

• D-Biotin 0.0035 mg/l
• Choline Chloride 8.98 mg/l
• D-Calcium Pantothenate 2.24 mg/l
• Folic Acid 2.66 mg/l
• myo-Inositol 12.60 mg/l
• Nicotinamide 2.02 mg/l
• Pyridoxine HCl 0.31 mg/l
• Riboflavin 0.22 mg/l
• Thiamine HCl 2.17 mg/l
• Vitamin B12 0.68 mg/l

Inorganic Salts

• CaCl2 2 H2O 154.40 mg/l
• CuSO4 5 H2O 0.0013 mg/l
• Fe(NO3)3 9 H2O 0.05 mg/l
• FeSO4 7 H2O 0.417 mg/l
• KCl 311.80 mg/l
• MgCl2 6 H2O 61.20 mg/l
• MgSO4 48.84 mg/l
• NaCl 6996.00 mg/l
• NaHCO3 2438.00 mg/l
• Na2HPO4 71.02 mg/l
• NaH2PO4 54.30 mg/l
• ZnSO4 7 H2O 0.43 mg/l

Other Components

• D-Glucose 3151.00 mg/l
• HEPES 3574.50 mg/l
• Hypoxanthine 2.10 mg/l
• Linoleic Acid 0.04 mg/l
• Lipoic Acid 0.105 mg/l
• Phenol Red Sodium Salt 8.63 mg/l
• Putrescine 2 HCl 0.081 mg/l
• Sodium Pyruvate 55.00 mg/l
• Tymidine 0.365 mg/l