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Colcemid Solution (10 µg/ml) in DPBS
N-Deacetyl-N-methylcolchicine (Colcemid) is related to colchicine but it is less toxic. Colcemid depolymerizes microtubules and limits their formation. Appropriate dilutions inactivate the spindle fiber formation, thus arresting cells in metaphase and allowing cell harvest and karyotyping to be performed.
Product Specifications
Appearance
Clear liquid
Shipping Conditions
Ambient
Working Concentration
Recommended final concentration: 0.1 µg/ml
Applications and Features
- Arrests cells in metaphase
- For chromosome analysis during lymphocyte karyotyping or amniotic fluid cell chromosome analysis
Storage and Shelf Life
Store at +2 °C to +8 °C upon arrival. Once opened store at +2 °C to +8 °C and use within 4-6 weeks.
Formulation
- Demecolcine (Colcemid) 10.00 mg/l
- CaCl₂ 2H₂O 130.00 mg/l
- Cl₂Mg 6H₂O 100.00 mg/l
- KCl 200.00 mg/l
- KH2PO4 200.00 mg/l
- NaCl 8000.00 mg/l
- Na2HPO4 1150.00 mg/l
Precautions and Disclaimer
This product is for research use only