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Accutase Cell Detachment Solution
Accutase is a cell detachment solution of proteolytic and collagenolytic enzymes. It has been developed to meet the most demanding requirements for gentle and effective detachment of adherent cells. It is useful for the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware.
Product Specifications
Appearance
Clear, pale red to orange solution
Shipping Conditions
Frozen (Dry ice)
Working Concentration
1x (ready-to-use)
Description
The advantages of Accutase over the traditional Trypsin/EDTA treatment are that it is less damaging to cells leading to increased viability and no inactivation of the enzymes by inhibitors or serum is required. The Accutase solution does not contain mammalian or bacterial-derived products.
Applications and Features
- Gentle detachment of a vast variety of cells including very sensitive cell lines e.g. hESCs
- Routine cell passage
- Analysis of cell surface markers
- Quiescence assays by serum starvation
- Transformation assays by oncogene transfection
- Neural crest cell migration assays, tumor cell migration assays
- Cell proliferation, apoptosis
- Production scale-up (bioreactor)
- Flow cytometry
Storage and Shelf Life
Store at ≤-15 °C Once defrosted, store at +2 to +8 °C. Note: Do not thaw the product in a 37 °C water bath. After thawing, the Accutase solution may be stored for up to 2 months at +2 to +8 °C. Do not store at room temperature.
Formulation
- EDTA 4Na 220 mg/l
- KCl 200 mg/l
- KH2PO4 200 mg/l
- NaCl 8000 mg/l
- Na2HPO4 1150 mg/l
- Phenol red 3 mg/l
- Accutase enzymes 1 x
Precautions and Disclaimer
This product is for research use only. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.