Accutase Cell Detachment Solution | LT Biotech EU-Grade Cell Culture Reagents

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Product Specifications

Appearance

Clear, pale red to orange solution

Shipping Conditions

Frozen (Dry ice)

Working Concentration

1x (ready-to-use)

Description

The advantages of Accutase over the traditional Trypsin/EDTA treatment are that it is less damaging to cells leading to increased viability and no inactivation of the enzymes by inhibitors or serum is required. The Accutase solution does not contain mammalian or bacterial-derived products.

Applications and Features

Gentle detachment of a vast variety of cells including very sensitive cell lines e.g. hESCs
Routine cell passage
Analysis of cell surface markers
Quiescence assays by serum starvation
Transformation assays by oncogene transfection
Neural crest cell migration assays, tumor cell migration assays
Cell proliferation, apoptosis
Production scale-up (bioreactor)
Flow cytometry

Storage and Shelf Life

Store at ≤-15 °C Once defrosted, store at +2 to +8 °C. Note: Do not thaw the product in a 37 °C water bath. After thawing, the Accutase solution may be stored for up to 2 months at +2 to +8 °C. Do not store at room temperature.

Download Data Sheet

Formulation

EDTA 4Na 220 mg/l
KCl 200 mg/l
KH2PO4 200 mg/l
NaCl 8000 mg/l
Na2HPO4 1150 mg/l
Phenol red 3 mg/l
Accutase enzymes 1 x

Precautions and Disclaimer

This product is for research use only. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.